IL12RB1 Kit ELISA

N° du produit ABIN2043799

Kit ELISA Humain IL12RB1, test Colorimetric pour la quantification de Humain IL12RB1.

Aperçu rapide pour IL12RB1 Kit ELISA (ABIN2043799)

Antigène: IL12RB1 (Interleukin 12 Receptor beta 1 (IL12RB1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.15-10 ng/mL

Application: ELISA

Détail du produit IL12RB1 Kit ELISA

Seuil minimal de détection: 0.15 ng/mL

Marque: BioAssay™

Sensibilité: < 0.071 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Interleukin 12 Receptor Beta 1 (IL12Rb1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 12 Receptor Beta 1 (IL12Rb1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 12 Receptor Beta 1 (IL12Rb1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 12 Receptor Beta 1 (IL12Rb1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Interleukin 12 Receptor Beta 1 (IL12Rb1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: IL12RB1 (Interleukin 12 Receptor beta 1 (IL12RB1))

Pathways: Signalistation JAK/STAT, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation