IL12B Kit ELISA

N° du produit ABIN2043801

Ce kit ELISA Porc IL12B est un kit ELISA Colorimetric conçu pour quantifier Porc IL12B.

Aperçu rapide pour IL12B Kit ELISA (ABIN2043801)

Antigène: IL12B (Interleukin 12 beta (IL12B))

Reactivité: Porc

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 7.81-500 pg/mL

Application: ELISA

Détail du produit IL12B Kit ELISA

Seuil minimal de détection: 7.81 pg/mL

Sensibilité: < 2.8 pg/mL

Information d'application

Plaque: Pre-coated

Protocole: Interleukin 12B (IL12B) BioAssay-ELISA Kit (Porcine) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 12B (IL12B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 12B (IL12B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 12B (IL12B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Interleukin 12B (IL12B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: IL12B (Interleukin 12 beta (IL12B))

Autre désignation: Interleukin 12B (IL12B)

Pathways: Signalistation JAK/STAT, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation