IL-4 Kit ELISA

N° du produit ABIN2043811

Kit ELISA IL-4 Singe, Colorimetric test pour la quantification de Singe IL-4.

Aperçu rapide pour IL-4 Kit ELISA (ABIN2043811)

Antigène: IL-4 (IL4) (Interleukin 4 (IL4))

Reactivité: Singe

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 3.12-200 pg/mL

Application: ELISA

Détail du produit IL-4 Kit ELISA

Seuil minimal de détection: 3.12 pg/mL

Sensibilité: < 1.26 pg/mL

Information d'application

Plaque: Pre-coated

Protocole: Interleukin 4 (IL4) BioAssay-ELISA Kit (Monkey) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 4 (IL4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 4 (IL4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 4 (IL4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Interleukin 4 (IL4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: IL-4 (IL4) (Interleukin 4 (IL4))

Pathways: Signalistation JAK/STAT, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation