JAM2 Kit ELISA

N° du produit ABIN2043831

Détail du produit JAM2 Kit ELISA

Antigène: JAM2 (Junctional Adhesion Molecule 2 (JAM2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2-2000 pg/mL

Seuil minimal de détection: 31.2 pg/mL

Application: ELISA

Marque: BioAssay™

Analytical Method: Quantitative

Sensibilité: 13.3 pg/mL

Ingrédients: A: Microtiter Strip, 1x96 wells, Pre-coated, ready to use
B: Standard, 2x1vial
C: Standard Diluent, 1x20 mL
D: Detection Reagent A (green), 1x120 µL
E: Detection Reagent B (red), 1x120 µL
F: Assay Diluent A, 1x10 mL
G: Assay Diluent B, 1x10 mL
H: TMB Substrate, 1x0 mL
K: Stop Solution, 1x0 mL
L: Wash Buffer, 30X, 1x20 mL

Information d'application

Plaque: Pre-coated

Protocole: The Human Junctional Adhesion Molecule 2 (JAM2) ELISA Kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of JAM2 in human serum, plasma, tissue homogenates and other biological fluids.The Microtiter Strip provided in this kit has been pre-coated with an antibody specific to JAM2. Standards or samples are then added to the appropriate Microtiter Strip wells with a biotin-conjugated antibody specific to JAM2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain JAM2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of JAM2 in the sample is then determined by comparing the O.D. of the sample to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: JAM2 (Junctional Adhesion Molecule 2 (JAM2))

Abstract: JAM2 Produits

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