Cytokeratin 18 Kit ELISA

N° du produit ABIN2043851

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Porc Cytokeratin 18.

Aperçu rapide pour Cytokeratin 18 Kit ELISA (ABIN2043851)

Antigène: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Reactivité: Porc

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.15-10 ng/mL

Application: ELISA

Détail du produit Cytokeratin 18 Kit ELISA

Seuil minimal de détection: 0.15 ng/mL

Sensibilité: < 0.054 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Keratin 18 (KRT18) BioAssay-ELISA Kit (Porcine) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Keratin 18 (KRT18). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Keratin 18 (KRT18). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Keratin 18 (KRT18), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Keratin 18 (KRT18) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Pathways: Apoptose, Caspase Cascade in Apoptosis