LIFR Kit ELISA

N° du produit ABIN2043887

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Humain LIFR.

Aperçu rapide pour LIFR Kit ELISA (ABIN2043887)

Antigène: LIFR (Leukemia Inhibitory Factor Receptor alpha (LIFR))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.31-20 ng/mL

Application: ELISA

Détail du produit LIFR Kit ELISA

Seuil minimal de détection: 0.31 ng/mL

Marque: BioAssay™

Sensibilité: < 0.113 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Leukemia Inhibitory Factor Receptor (LIFR) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Leukemia Inhibitory Factor Receptor (LIFR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Leukemia Inhibitory Factor Receptor (LIFR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Leukemia Inhibitory Factor Receptor (LIFR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Leukemia Inhibitory Factor Receptor (LIFR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: LIFR (Leukemia Inhibitory Factor Receptor alpha (LIFR))

Autre désignation: Leukemia Inhibitory Factor Receptor (LIFR)

Pathways: Signalistation JAK/STAT, Growth Factor Binding