Lipase A Kit ELISA

N° du produit ABIN2043893

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Souris Lipase A.

Aperçu rapide pour Lipase A Kit ELISA (ABIN2043893)

Antigène: Lipase A (LIPA) (Lipase A, Lysosomal Acid, Cholesterol Esterase (LIPA))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.31-20 ng/mL

Application: ELISA

Détail du produit Lipase A Kit ELISA

Seuil minimal de détection: 0.31 ng/mL

Sensibilité: < 0.117 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Lipase A, Lysosomal Acid (LIPA) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lipase A, Lysosomal Acid (LIPA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lipase A, Lysosomal Acid (LIPA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lipase A, Lysosomal Acid (LIPA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Lipase A, Lysosomal Acid (LIPA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: Lipase A (LIPA) (Lipase A, Lysosomal Acid, Cholesterol Esterase (LIPA))

Autre désignation: Lipase A, Lysosomal Acid (LIPA)