PPARA Kit ELISA

N° du produit ABIN2044205

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Rat PPARA.

Aperçu rapide pour PPARA Kit ELISA (ABIN2044205)

Antigène: PPARA (Peroxisome Proliferator-Activated Receptor alpha (PPARA))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.31-20 ng/mL

Application: ELISA

Détail du produit PPARA Kit ELISA

Seuil minimal de détection: 0.31 ng/mL

Sensibilité: < 0.113 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Peroxisome Proliferator Activated Receptor Alpha (PPARa) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peroxisome Proliferator Activated Receptor Alpha (PPARa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peroxisome Proliferator Activated Receptor Alpha (PPARa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peroxisome Proliferator Activated Receptor Alpha (PPARa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Peroxisome Proliferator Activated Receptor Alpha (PPARa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: PPARA (Peroxisome Proliferator-Activated Receptor alpha (PPARA))

Autre désignation: Peroxisome Proliferator Activated Receptor alpha (PPARa)

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha, Regulation of Carbohydrate Metabolic Process, Hepatitis C