PDE1A Kit ELISA

N° du produit ABIN2044213

Kit ELISA Humain PDE1A, test Colorimetric pour la quantification de Humain PDE1A.

Aperçu rapide pour PDE1A Kit ELISA (ABIN2044213)

Antigène: PDE1A (Phosphodiesterase 1A (PDE1A))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.31-20 ng/mL

Application: ELISA

Détail du produit PDE1A Kit ELISA

Seuil minimal de détection: 0.31 ng/mL

Sensibilité: < 0.126 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Phosphodiesterase 1A, Calmodulin Dependent (PDE1A) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphodiesterase 1A, Calmodulin Dependent (PDE1A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phosphodiesterase 1A, Calmodulin Dependent (PDE1A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphodiesterase 1A, Calmodulin Dependent (PDE1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Phosphodiesterase 1A, Calmodulin Dependent (PDE1A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: PDE1A (Phosphodiesterase 1A (PDE1A))

Autre désignation: Phosphodiesterase 1A, Calmodulin Dependent (PDE1A)

Pathways: Neurotrophin Signaling Pathway, cAMP Metabolic Process, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma