PLA2G4D Kit ELISA

N° du produit ABIN2044219

Kit ELISA PLA2G4D Humain, Colorimetric test pour la quantification de Humain PLA2G4D.

Aperçu rapide pour PLA2G4D Kit ELISA (ABIN2044219)

Antigène: PLA2G4D (phospholipase A2, Group IVD (Cytosolic) (PLA2G4D))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.15-10 ng/mL

Application: ELISA

Détail du produit PLA2G4D Kit ELISA

Seuil minimal de détection: 0.15 ng/mL

Sensibilité: < 0.064 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Phospholipase A2, Group IVD (PLA2G4D) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase A2, Group IVD (PLA2G4D). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phospholipase A2, Group IVD (PLA2G4D). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospholipase A2, Group IVD (PLA2G4D), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Phospholipase A2, Group IVD (PLA2G4D) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: PLA2G4D (phospholipase A2, Group IVD (Cytosolic) (PLA2G4D))

Autre désignation: Phospholipase A2, Group IVD (PLA2G4D)

Pathways: Inositol Metabolic Process, VEGF Signaling