F2RL3 Kit ELISA
Aperçu rapide pour F2RL3 Kit ELISA (ABIN2044313)
Antigène
Voir toutes F2RL3 Kits ELISAReactivité
Méthode de détection
Type de méthode
Gamme de detection
Application
-
-
Seuil minimal de détection
- 0.15 ng/mL
-
Sensibilité
- < 0.059 ng/mL
-
-
-
-
Plaque
- Pre-coated
-
Protocole
- Protease Activated Receptor 4 (PAR4) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protease Activated Receptor 4 (PAR4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protease Activated Receptor 4 (PAR4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protease Activated Receptor 4 (PAR4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Protease Activated Receptor 4 (PAR4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
-
Restrictions
- For Research Use only
-
-
-
Stock
- 4 °C/-20 °C
-
-
- F2RL3 (Coagulation Factor II (Thrombin) Receptor-Like 3 (F2RL3))
-
Autre désignation
- Protease Activated Receptor 4 (PAR4)
-
Pathways
- Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process
Antigène Voir toutes F2RL3 Kits ELISA
-