F2RL3 Kit ELISA

N° du produit ABIN2044313

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Rat F2RL3.

Aperçu rapide pour F2RL3 Kit ELISA (ABIN2044313)

Antigène: F2RL3 (Coagulation Factor II (Thrombin) Receptor-Like 3 (F2RL3))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.15-10 ng/mL

Application: ELISA

Détail du produit F2RL3 Kit ELISA

Seuil minimal de détection: 0.15 ng/mL

Sensibilité: < 0.059 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Protease Activated Receptor 4 (PAR4) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protease Activated Receptor 4 (PAR4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protease Activated Receptor 4 (PAR4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protease Activated Receptor 4 (PAR4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Protease Activated Receptor 4 (PAR4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: F2RL3 (Coagulation Factor II (Thrombin) Receptor-Like 3 (F2RL3))

Autre désignation: Protease Activated Receptor 4 (PAR4)

Pathways: Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process