SUMO1 Kit ELISA
Aperçu rapide pour SUMO1 Kit ELISA (ABIN2044567)
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Méthode de détection
Type de méthode
Gamme de detection
Application
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Seuil minimal de détection
- 0.15 ng/mL
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Sensibilité
- < 0.061 ng/mL
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Plaque
- Pre-coated
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Protocole
- Small Ubiquitin Related Modifier Protein 1 (SUMO1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Small Ubiquitin Related Modifier Protein 1 (SUMO1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Small Ubiquitin Related Modifier Protein 1 (SUMO1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Small Ubiquitin Related Modifier Protein 1 (SUMO1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Small Ubiquitin Related Modifier Protein 1 (SUMO1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Restrictions
- For Research Use only
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Stock
- 4 °C/-20 °C
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- SUMO1 (Small Ubiquitin Related Modifier Protein 1 (SUMO1))
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Pathways
- M Phase, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus, Ubiquitin Proteasome Pathway
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