CCL17 Kit ELISA
Aperçu rapide pour CCL17 Kit ELISA (ABIN2044665)
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Méthode de détection
Type de méthode
Gamme de detection
Application
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Seuil minimal de détection
- 31.25 pg/mL
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Marque
- BioAssay™
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Sensibilité
- < 10.5 pg/mL
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Plaque
- Pre-coated
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Protocole
- Thymus Activation Regulated Chemokine (TARC) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thymus Activation Regulated Chemokine (TARC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thymus Activation Regulated Chemokine (TARC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thymus Activation Regulated Chemokine (TARC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Thymus Activation Regulated Chemokine (TARC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Restrictions
- For Research Use only
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Stock
- 4 °C/-20 °C
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- CCL17 (Chemokine (C-C Motif) Ligand 17 (CCL17))
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Autre désignation
- Thymus Activation Regulated Chemokine (TARC)
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