UBE2I Kit ELISA

N° du produit ABIN2044739

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Humain UBE2I.

Aperçu rapide pour UBE2I Kit ELISA (ABIN2044739)

Antigène: UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.15-10 ng/mL

Application: ELISA

Détail du produit UBE2I Kit ELISA

Seuil minimal de détection: 0.15 ng/mL

Sensibilité: < 0.053 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Ubiquitin Conjugating Enzyme E2I (UBE2I) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquitin Conjugating Enzyme E2I (UBE2I). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ubiquitin Conjugating Enzyme E2I (UBE2I). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ubiquitin Conjugating Enzyme E2I (UBE2I), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Ubiquitin Conjugating Enzyme E2I (UBE2I) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))

Autre désignation: Ubiquitin Conjugating Enzyme E2I (UBE2I)

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Ubiquitin Proteasome Pathway