VASP Kit ELISA

N° du produit ABIN2044785

Ce kit ELISA Rat VASP est un kit ELISA Colorimetric conçu pour quantifier Rat VASP.

Aperçu rapide pour VASP Kit ELISA (ABIN2044785)

Antigène: VASP (Vasodilator-Stimulated phosphoprotein (VASP))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.15-10 ng/mL

Application: ELISA

Détail du produit VASP Kit ELISA

Seuil minimal de détection: 0.15 ng/mL

Sensibilité: < 0.052 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Vasodilator Stimulated Phosphoprotein (VASP) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vasodilator Stimulated Phosphoprotein (VASP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vasodilator Stimulated Phosphoprotein (VASP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vasodilator Stimulated Phosphoprotein (VASP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Vasodilator Stimulated Phosphoprotein (VASP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: VASP (Vasodilator-Stimulated phosphoprotein (VASP))

Autre désignation: Vasodilator Stimulated Phosphoprotein (VASP)

Pathways: TCR Signaling, Regulation of Actin Filament Polymerization, Tube Formation