WASF2 Kit ELISA
Aperçu rapide pour WASF2 Kit ELISA (ABIN2044845)
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Méthode de détection
Type de méthode
Gamme de detection
Application
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Seuil minimal de détection
- 0.15 ng/mL
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Sensibilité
- < 0.053 ng/mL
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Plaque
- Pre-coated
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Protocole
- Wiskott Aldrich Syndrome Protein Family, Member 2 (WASF2) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wiskott Aldrich Syndrome Protein Family, Member 2 (WASF2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Wiskott Aldrich Syndrome Protein Family, Member 2 (WASF2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Wiskott Aldrich Syndrome Protein Family, Member 2 (WASF2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Wiskott Aldrich Syndrome Protein Family, Member 2 (WASF2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Restrictions
- For Research Use only
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Stock
- 4 °C/-20 °C
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- WASF2 (WAS Protein Family, Member 2 (WASF2))
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Autre désignation
- Wiskott Aldrich Syndrome Protein Family, Member 2 (WASF2)
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Pathways
- Signalisation RTK
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