YAP1 Kit ELISA

N° du produit ABIN2044861

Kit ELISA YAP1 Souris, Colorimetric test pour la quantification de Souris YAP1.

Aperçu rapide pour YAP1 Kit ELISA (ABIN2044861)

Antigène: YAP1 (Yes-Associated Protein 1 (YAP1))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.15-10 ng/mL

Application: ELISA

Détail du produit YAP1 Kit ELISA

Seuil minimal de détection: 0.15 ng/mL

Sensibilité: < 0.056 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Yes Associated Protein 1 (YAP1) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Yes Associated Protein 1 (YAP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Yes Associated Protein 1 (YAP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Yes Associated Protein 1 (YAP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Yes Associated Protein 1 (YAP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: YAP1 (Yes-Associated Protein 1 (YAP1))

Autre désignation: Yes Associated Protein 1 (YAP1)

Pathways: Signalisation MAPK, Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha