ZP2 Kit ELISA

N° du produit ABIN2044869

Le kit ELISA ZP2 (ABIN2044869) Rat est un kit ELISA Colorimetric conçu pour quantifier Rat ZP2.

Aperçu rapide pour ZP2 Kit ELISA (ABIN2044869)

Antigène: ZP2 (Zona Pellucida Glycoprotein 2 (ZP2))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.31-20 ng/mL

Application: ELISA

Détail du produit ZP2 Kit ELISA

Seuil minimal de détection: 0.31 ng/mL

Sensibilité: < 0.125 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Zona Pellucida Glycoprotein 2, Sperm Receptor (ZP2) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Zona Pellucida Glycoprotein 2, Sperm Receptor (ZP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Zona Pellucida Glycoprotein 2, Sperm Receptor (ZP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Zona Pellucida Glycoprotein 2, Sperm Receptor (ZP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Zona Pellucida Glycoprotein 2, Sperm Receptor (ZP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: ZP2 (Zona Pellucida Glycoprotein 2 (ZP2))

Autre désignation: Zona Pellucida Glycoprotein 2, Sperm Receptor (ZP2)