GM-CSF Kit ELISA

N° du produit ABIN2111989

Kit ELISA Souris GM-CSF, test Colorimetric pour la quantification de Souris GM-CSF.

Aperçu rapide pour GM-CSF Kit ELISA (ABIN2111989)

Antigène: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 50-3200 pg/mL

Application: ELISA

Détail du produit GM-CSF Kit ELISA

Seuil minimal de détection: 50 pg/mL

Analytical Method: Quantitative

Sensibilité: < 17 pg/mL

Ingrédients: MOUSE GM-CSF MICROTITER PLATE
BIOTIN CONJUGATE
AVIDIN CONJUGATE
MOUSE GM-CSF STANDARD
CALIBRATOR DILUENT I
CALIBRATOR DILUENT II
WASH BUFFER (20X/96 wells, 30X/192 wells)
SUBSTRATE A
SUBSTRATE B
STOP SOLUTION

Information d'application

Plaque: Pre-coated

Protocole: This ELISA kit provides a tool for studying GM-CSF expression and regulation in animal model.This mouse GM-CSF enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for mouse GM-CSF. Standards or samples are then added to the appropriate microtiter plate wells and incubated. Mouse GM-CSF, if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove unbound mouse GM- CSF and other components of the sample. In order to quantitatively determine the amount of mouse GM-CSF present in the sample, a standardized preparation of horseradish peroxidase HRP-conjugated monoclonal antibody specific for mouse GM-CSF is added to each well to -sandwich- the mouse GM-CSF immobilized during the first incubation. The microtiter plate then undergoes a second incubation. The wells are thoroughly washed to remove all unbound HRP-conjugated antibodies and a TMB (3,3-5,5- tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain mouse GM-CSF and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 ± 2nm.

Restrictions: For Research Use only

Stockage

Détail du antigène

Antigène: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Autre désignation: Granulocyte Macrophage Colony Stimulating Factor

Pathways: Signalistation JAK/STAT, Cellular Response to Molecule of Bacterial Origin