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LAT Kit ELISA

Ce kit ELISA Humain LAT est un kit ELISA Colorimetric conçu pour quantifier Humain LAT.
N° du produit ABIN2535943

Aperçu rapide pour LAT Kit ELISA (ABIN2535943)

Antigène

Voir toutes LAT Kits ELISA
LAT (Linker For Activation of T Cells (LAT))

Reactivité

  • 7
  • 4
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Humain

Méthode de détection

Colorimetric

Type de méthode

Sandwich ELISA

Gamme de detection

62.5 pg/mL - 4000 pg/mL

Application

ELISA

Type d'échantillon

Cell Lysate, Plasma, Serum, Tissue Homogenate
  • Seuil minimal de détection

    62.5 pg/mL

    Fonction

    Human Linker for activation of T-cells family member 1 (LAT) ELISA Kit

    Analytical Method

    Quantitative

    Sensibilité

    37.5 pg/mL

    Ingrédients

    The kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.
    • Pre-coated 96-Well Microplate
    • Standard
    • Standard Diluent Buffer
    • Wash Buffer
    • Detection Reagent A
    • Detection Reagent B
    • Diluent A
    • Diluent B
    • TMB Substrate
    • Stop Solution
    • Plate Sealer

    Matériel non inclus

    • 37 °C incubator
    • Multi and single channel pipettes and sterile pipette tips
    • Squirt bottle or automated microplate washer
    • 1.5 mL tubes
    • Distilled water
    • Absorbent filter papers
    • 100 mL and 1 liter graduated cylinders
    • Microplate reader (wavelength: 450 nm)
    • ELISA Shaker
  • Indications d'application

    Optimal dilutions/concentrations should be determined by the end user.

    Commentaires

    The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5 % within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.

    Plaque

    Pre-coated

    Précision du teste

    Intra-assay Precision (Precision within an assay): 3 samples with low, medium and high levels of Linker for activation of T-cells family member 1 (LAT) were tested 20 times on one plate, respectively.

    Inter-assay Precision (Precision between assays): 3 samples with low, medium and high levels of Linker for activation of T-cells family member 1 (LAT) were tested on 3 different plates, 8 replicates in each plate.

    CV (%) = (Standard Deviation / mean) x 100

    Intra-Assay: CV<10%

    Inter-Assay: CV<10%

    Restrictions

    For Research Use only
  • Stock

    4 °C

    Stockage commentaire

    Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.

    Date de péremption

    6 months
  • Antigène Voir toutes LAT Kits ELISA

    LAT (Linker For Activation of T Cells (LAT))

    Autre désignation

    Linker for activation of T-cells family member 1

    Pathways

    TCR Signaling, Fc-epsilon Receptor Signaling Pathway
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