IL4 Receptor Kit ELISA
Aperçu rapide pour IL4 Receptor Kit ELISA (ABIN2648537)
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Méthode de détection
Application
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Fonction
- Human IL-4R ELISA is to be used for the in-vitro quantitative determination of soluble IL-4 receptor molecule (CD124) in human serum, plasma, buffered solutions or cell culture medium. The assay will recognize both natural and recombinant human IL-4R.
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Analytical Method
- Quantitative
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Attributs du produit
- Human IL-4R ELISA Assay kit is for Research Use Only.
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Indications d'application
- Optimal working dilution should be determined by the investigator.
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Plaque
- Pre-coated
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Protocole
- The IL-4R Kit is a solid phase sandwich Enzyme Linked-Immuno- Sorbent Assay (ELISA). A monoclonal antibody specific for IL-4R has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-4R concentrations and unknowns are pipetted into these wells. During the first incubation, the IL-4R antigen and a biotinylated monoclonal antibody specific for IL-4R are simultaneously incubated. After washing, the enzyme ( streptavidin-peroxydase ) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-4R present in the samples.
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Restrictions
- For Research Use only
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Stock
- 4 °C
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- IL4 Receptor (IL4R) (Interleukin 4 Receptor (IL4R))
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Autre désignation
- CD124 / IL-4R
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Pathways
- Signalistation JAK/STAT, Positive Regulation of Immune Effector Process
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