S100A8/A9 Complex (Calprotectin) Kit ELISA

N° du produit ABIN2648553

Ce kit ELISA Humain S100A8/A9 Complex (Calprotectin) est un kit ELISA Colorimetric conçu pour quantifier Humain S100A8/A9 Complex (Calprotectin).

Aperçu rapide pour S100A8/A9 Complex (Calprotectin) Kit ELISA (ABIN2648553)

Antigène: S100A8/A9 Complex (Calprotectin) (S100A8/A9) (Calprotectin (S100A8/A9))

Reactivité: Humain

Méthode de détection: Colorimetric

Application: ELISA

Type d'échantillon: Fecal

Détail du produit S100A8/A9 Complex (Calprotectin) Kit ELISA

Sensibilité: 5 ng/ml

Attributs du produit: This Calprotectin ELISA Assay kit is intended for use in the quantitative determination of human calprotectin (neutrophil cytoplasmic protein S100A8/A9, MRP 8/14) levels in stool samples via Enzyme-linked immunosorbent assay. The Calprotectin ELISA Assay kit is for research use only and should not be used in diagnostic procedures.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Volume d'échantillon: 50 mg

Durée du test: 2 h

Protocole: Assay standards, controls and patient samples are added directly to wells of a microtiter plate that is coated with antibody to calprotectin. After a short incubation period, the plate is washed and horseradish peroxidase (HRP) conjugated human calprotectin specific monoclonal antibody is added to each well. After the second incubation period, a "sandwich" of solid-phase antibody - human calprotectin - HRP conjugated monoclonal antibody" is formed. The unbound monoclonal antibodies and buffer matrix are removed in the subsequent washing step. For the detection of this immunocomplex, the well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to the wall of each microtiter well is directly proportional to the amount of human calprotectin in the test sample. A standard curve is generated by plotting the absorbance versus the respective human calprotectin concentration for each standard on a point-to-point or 4-parameter curve fitting. The concentration of fecal human calprotectin in test samples is determined directly from this standard curve of the Calprotectin ELISA Assay.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Détail du antigène

Antigène: S100A8/A9 Complex (Calprotectin) (S100A8/A9) (Calprotectin (S100A8/A9))

Autre désignation: Calprotectin

Pathways: S100 Proteins