IL-2 Kit ELISA
Aperçu rapide pour IL-2 Kit ELISA (ABIN2648691)
Antigène
Voir toutes IL-2 (IL2) Kits ELISAReactivité
Méthode de détection
Application
Type d'échantillon
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Fonction
- Human Interleukin 2 (IL-2) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Interleukin 2 (IL-2) concentrations in cell culture supernates, serum, and plasma.
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Analytical Method
- Quantitative
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Sensibilité
- 2 pg/ml
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Attributs du produit
- Human Interleukin 2 (IL-2) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.
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Interleukin 2 (IL2) ELISA Kit
IL2 Reactivité: Humain Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 2 (IL2) ELISA KitHigh Sensitivity IL2 Reactivité: Humain Colorimetric Sandwich ELISA 1.56 pg/mL - 100 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 2 (IL2) ELISA KitIL2 Reactivité: Souris AA 21-169 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Interleukin 2 (IL2) ELISA KitIL2 Reactivité: Rat Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Serum, Tissue Homogenate, Urine
Interleukin 2 (IL2) ELISA KitIL2 Reactivité: Souris Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 2 (IL2) ELISA KitIL2 Reactivité: Rat Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 2 (IL2) ELISA KitIL2 Reactivité: Lapin Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 2 (IL2) ELISA KitIL2 Reactivité: Poulet Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 2 (IL2) ELISA KitIL2 Reactivité: Chien Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 2 (IL2) ELISA KitIL2 Reactivité: Porc Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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Indications d'application
- Optimal working dilution should be determined by the investigator.
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Volume d'échantillon
- 100 μL
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Durée du test
- 3.5 h
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Plaque
- Pre-coated
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Protocole
- This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-2 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-2 is added to the wells and binds to the combination of capture antibody-IL-2 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IL-2 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-2 standard dilutions and IL-2 sample concentration determined.
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Restrictions
- For Research Use only
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Stock
- 4 °C
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- IL-2 (IL2) (Interleukin 2 (IL2))
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Autre désignation
- IL-2
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Pathways
- Signalistation JAK/STAT, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
Antigène Voir toutes IL-2 (IL2) Kits ELISA
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