IL-32 alpha Kit ELISA
Aperçu rapide pour IL-32 alpha Kit ELISA (ABIN2648765)
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Méthode de détection
Application
Type d'échantillon
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Fonction
- Human Interleukin 32 Alpha (IL-32α) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Interleukin 32 Alpha (IL-32α) concentrations in cell culture supernates, serum, and plasma.
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Analytical Method
- Quantitative
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Sensibilité
- 7 pg/ml
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Attributs du produit
- Human Interleukin 32 Alpha (IL-32α) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.
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Indications d'application
- Optimal working dilution should be determined by the investigator.
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Volume d'échantillon
- 100 μL
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Durée du test
- 3.5 h
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Plaque
- Pre-coated
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Protocole
- Human Interleukin 32 Alpha (IL-32α) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-32α ? has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-32α present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-32α is added to the wells and binds to the combination of capture antibody- IL-32α in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IL-32α present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-32α standard dilutions and IL-32α sample concentration determined.
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Restrictions
- For Research Use only
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Stock
- 4 °C
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- IL-32 alpha (IL32A) (Interleukin 32 alpha (IL32A))
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Autre désignation
- IL-32a
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