GM-CSF Kit ELISA

N° du produit ABIN2648793

Ce kit ELISA Humain GM-CSF est un kit ELISA Colorimetric conçu pour quantifier Humain GM-CSF.

Aperçu rapide pour GM-CSF Kit ELISA (ABIN2648793)

Antigène: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Reactivité: Humain

Méthode de détection: Colorimetric

Application: ELISA

Type d'échantillon: Serum, Plasma, Cell Culture Cells

Détail du produit GM-CSF Kit ELISA

Fonction: Human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) concentrations in cell culture supernates, serum, and plasma.

Analytical Method: Quantitative

Sensibilité: 7 pg/ml

Attributs du produit: Human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Volume d'échantillon: 100 μL

Durée du test: 3 h

Plaque: Pre-coated

Protocole: This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for GM-CSF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GM-CSF present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for GM-CSF is added to the wells and binds to the combination of capture antibody- GM-CSF in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of GM-CSF present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven GM-CSF standard dilutions and GM-CSF sample concentration determined.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Détail du antigène

Antigène: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Autre désignation: GM-CSF

Pathways: Signalistation JAK/STAT, Cellular Response to Molecule of Bacterial Origin