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C5A Kit ELISA

C5a Reactivité: Humain Colorimetric Sandwich ELISA Plasma (EDTA), Serum
N° du produit ABIN2690553
  • Antigène Voir toutes C5A (C5a) Kits ELISA
    C5A (C5a) (Complement Component 5a (C5a))
    Reactivité
    • 7
    • 6
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Seuil minimal de détection
    0.047 ng/mL
    Application
    ELISA
    Fonction
    The Human C5a ELISA Kit II is for the in vitro quantitative determination of Human C5a-desArg in human EDTA plasma, serum and other biological samples.
    Marque
    BD OptEIA™
    Type d'échantillon
    Plasma (EDTA), Serum
    Analytical Method
    Quantitative
    Ingrédients
    Antibody Coated Wells - 2 plates of 96 breakable wells (12 strips x 8 wells per plate)
    Detection Antibody - 30 mL
    Standards - 4 vials, lyophilized
    Enzyme Concentrate (250x) - 150 μL
    Standard/Sample Diluent - 30 mL
    ELISA Diluent - 12 mL
    Wash Concentrate (20x) - 100 mL
    TMB One-Step Substrate Reagent - 30 mL
    Stop Solution - 13 mL
    Plate Sealers - 4 each
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  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Commentaires

    BD OptEIA™ Human C5a ELISA Kit II

    Plaque
    Pre-coated
    Restrictions
    For Research Use only
  • Stock
    4 °C
    Stockage commentaire
    1. Store kit at 2 - 8°C. Do not use kit after expiration date.
    2. Before use, bring all reagents to room temperature (18 - 25°C). Immediately after use, return to proper storage conditions.
    3. Lyophilized standards are stable until kit expiration date. After reconstitution, use freshly reconstituted standard within 12 hours (stored at 2 - 8°C).
  • Pfeifer, Kawahara, Hugli: "Possible mechanism for in vitro complement activation in blood and plasma samples: futhan/EDTA controls in vitro complement activation." dans: Clinical chemistry, Vol. 45, Issue 8 Pt 1, pp. 1190-9, (1999) (PubMed).

    Matsushita, Fujita: "Activation of the classical complement pathway by mannose-binding protein in association with a novel C1s-like serine protease." dans: The Journal of experimental medicine, Vol. 176, Issue 6, pp. 1497-502, (1993) (PubMed).

    Hugli, Müller-Eberhard: "Anaphylatoxins: C3a and C5a." dans: Advances in immunology, Vol. 26, pp. 1-53, (1978) (PubMed).

    Bokisch, Müller-Eberhard: "Anaphylatoxin inactivator of human plasma: its isolation and characterization as a carboxypeptidase." dans: The Journal of clinical investigation, Vol. 49, Issue 12, pp. 2427-36, (1971) (PubMed).

  • Antigène Voir toutes C5A (C5a) Kits ELISA
    C5A (C5a) (Complement Component 5a (C5a))
    Autre désignation
    C5a (C5a Produits)
    Synonymes
    C5a Kit ELISA, C5b Kit ELISA, CPAMD4 Kit ELISA, Hc Kit ELISA, RGD1561905 Kit ELISA, complement C5 Kit ELISA, C5 Kit ELISA
    Pathways
    Système du Complément, Carbohydrate Homeostasis
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