C5A Kit ELISA
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- Antigène Voir toutes C5A (C5a) Kits ELISA
- C5A (C5a) (Complement Component 5a (C5a))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Seuil minimal de détection
- 0.047 ng/mL
- Application
- ELISA
- Fonction
- The Human C5a ELISA Kit II is for the in vitro quantitative determination of Human C5a-desArg in human EDTA plasma, serum and other biological samples.
- Marque
- BD OptEIA™
- Type d'échantillon
- Plasma (EDTA), Serum
- Analytical Method
- Quantitative
- Ingrédients
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Antibody Coated Wells - 2 plates of 96 breakable wells (12 strips x 8 wells per plate)
Detection Antibody - 30 mL
Standards - 4 vials, lyophilized
Enzyme Concentrate (250x) - 150 μL
Standard/Sample Diluent - 30 mL
ELISA Diluent - 12 mL
Wash Concentrate (20x) - 100 mL
TMB One-Step Substrate Reagent - 30 mL
Stop Solution - 13 mL
Plate Sealers - 4 each
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Commentaires
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BD OptEIA™ Human C5a ELISA Kit II
- Plaque
- Pre-coated
- Restrictions
- For Research Use only
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- Stock
- 4 °C
- Stockage commentaire
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1. Store kit at 2 - 8°C. Do not use kit after expiration date.
2. Before use, bring all reagents to room temperature (18 - 25°C). Immediately after use, return to proper storage conditions.
3. Lyophilized standards are stable until kit expiration date. After reconstitution, use freshly reconstituted standard within 12 hours (stored at 2 - 8°C).
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Possible mechanism for in vitro complement activation in blood and plasma samples: futhan/EDTA controls in vitro complement activation." dans: Clinical chemistry, Vol. 45, Issue 8 Pt 1, pp. 1190-9, (1999) (PubMed).
: "Activation of the classical complement pathway by mannose-binding protein in association with a novel C1s-like serine protease." dans: The Journal of experimental medicine, Vol. 176, Issue 6, pp. 1497-502, (1993) (PubMed).
: "Anaphylatoxins: C3a and C5a." dans: Advances in immunology, Vol. 26, pp. 1-53, (1978) (PubMed).
: "Anaphylatoxin inactivator of human plasma: its isolation and characterization as a carboxypeptidase." dans: The Journal of clinical investigation, Vol. 49, Issue 12, pp. 2427-36, (1971) (PubMed).
: "
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Possible mechanism for in vitro complement activation in blood and plasma samples: futhan/EDTA controls in vitro complement activation." dans: Clinical chemistry, Vol. 45, Issue 8 Pt 1, pp. 1190-9, (1999) (PubMed).
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- Antigène Voir toutes C5A (C5a) Kits ELISA
- C5A (C5a) (Complement Component 5a (C5a))
- Autre désignation
- C5a (C5a Produits)
- Synonymes
- C5a Kit ELISA, C5b Kit ELISA, CPAMD4 Kit ELISA, Hc Kit ELISA, RGD1561905 Kit ELISA, complement C5 Kit ELISA, C5 Kit ELISA
- Pathways
- Système du Complément, Carbohydrate Homeostasis
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