APOA1 Kit ELISA

N° du produit ABIN2702827

Ce kit ELISA Humain et Macaque rhésus APOA1 est un kit ELISA Colorimetric conçu pour quantifier Humain et Macaque rhésus APOA1.

Aperçu rapide pour APOA1 Kit ELISA (ABIN2702827)

Antigène: APOA1 (Apolipoprotein A-I (APOA1))

Reactivité: Humain, Macaque rhésus

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.08-20 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Détail du produit APOA1 Kit ELISA

Seuil minimal de détection: 0.08 ng/mL

Fonction: Human ApoA ELISA Kit for cell culture supernatants, plasma, and serum samples.

Analytical Method: Quantitative

Specificité: This ELISA antibody pair detects human ApoA1. Cross-reacts with ApoA1 from rhesus and cynomolgus macaques. Other species not determined.

Sensibilité: 0.08 ng/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples1,000,000 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Préparation de l'échantillon:

For example, add 1 μL of serum or plasma into a tube with 999 μL 1X Assay Diluent to prepare a 1,000-fold diluted sample. Mix thoroughly and then pipette 1 μL of prepared 1,000-fold diluted sample into a tube with 999 μL 1X Assay Diluent to prepare a final 1,000,000-fold diluted sample.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: APOA1 (Apolipoprotein A-I (APOA1))

Autre désignation: APOA1

Sujet: Gene Names: APOA1
Protein names: Apolipoprotein A-I (Apo-AI) (ApoA-I) (Apolipoprotein A1) [Cleaved into: Proapolipoprotein A-I (ProapoA-I), Truncated apolipoprotein A-I (Apolipoprotein A-I(1-242))]

ID gène: 335

UniProt: P02647

Pathways: Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response, Lipid Metabolism