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Apolipoprotein C-II Kit ELISA

APOC2 Reactivité: Humain Colorimetric Sandwich ELISA 1.5-100 ng/mL Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN2702831
  • Antigène Voir toutes Apolipoprotein C-II (APOC2) Kits ELISA
    Apolipoprotein C-II (APOC2)
    Reactivité
    • 7
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    1.5-100 ng/mL
    Seuil minimal de détection
    1.5 ng/mL
    Application
    ELISA
    Fonction
    Human ApoC2 ELISA Kit for cell culture supernatants, plasma, and serum samples.
    Type d'échantillon
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Quantitative
    Specificité
    Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human Alpha-2 Microglobulin, ApoA, ApoC1, ApoC3, ApoE, ApoH, Artemin, BMP-6, Calbindin, CK beta 8-1 (CCL23), CNTF, EMMPRIN (CD147), Endostatin, Fibrinogen, FLRG, TNFSF18 (GITR Ligand), GPBB, Hemoglobin, HTRA2, IL-17E, IL-1 R3 (IL1 R Acp), Lactoferrin, Mesothelin, PAPP-A, PD-ECGF (Thymidine Phosphorylase), PDGF R alpha, Pepsinogen 2, Periostin, Persephin, PTH, RAP, SAA (Serum Amyloid A), Serpin F1 (PEDF), Tie-1, TPA, Thrombospondin 2, Ubiquitin+1, Vitronectin, VWF
    Sensibilité
    1.5 ng/mL
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Indications d'application
    Recommended Dilution for serum and plasma samples20,000 fold
    Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Préparation de l'échantillon

    For example, add 2 μL of serum/plasma into a tube with 198.0 μL Assay Diluent C to prepare a 100-fold diluted sample. Mix thoroughly and then pipette 2 μL of prepared 100-fold diluted sample into a tube with 398 μL Assay Diluent C to prepare a final 20,000 fold diluted sample.

    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes Apolipoprotein C-II (APOC2) Kits ELISA
    Apolipoprotein C-II (APOC2)
    Autre désignation
    ApoC2 (APOC2 Produits)
    Synonymes
    apoc2 Kit ELISA, apoC-II Kit ELISA, fb98g08 Kit ELISA, wu:fb52e03 Kit ELISA, wu:fb71a04 Kit ELISA, wu:fb98g08 Kit ELISA, APOC2 Kit ELISA, APO-CII Kit ELISA, APOC-II Kit ELISA, RGD1560725 Kit ELISA, APOCII Kit ELISA, Apo-CII Kit ELISA, ApoC-II Kit ELISA, apolipoprotein C-II Kit ELISA, apolipoprotein C2 Kit ELISA, apoc2 Kit ELISA, APOC2 Kit ELISA, Apoc2 Kit ELISA
    Sujet
    Gene Names: APOC2 APC2
    Protein names: Apolipoprotein C-II (Apo-CII) (ApoC-II) (Apolipoprotein C2) [Cleaved into: Proapolipoprotein C-II (ProapoC-II)]
    ID gène
    344
    UniProt
    P02655
    Pathways
    Lipid Metabolism
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