BMP7 Kit ELISA

N° du produit ABIN2702870

Détail du produit BMP7 Kit ELISA

Antigène: BMP7 (Bone Morphogenetic Protein 7 (BMP7))

Reactivité: Humain, Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 4-1.000 pg/mL

Seuil minimal de détection: 4 pg/mL

Application: ELISA

Fonction: Mouse BMP-7 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificité: This ELISA antibody pair detects mouse and human BMP-7. Other species not determined.

Sensibilité: 4 pg/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples2 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: BMP7 (Bone Morphogenetic Protein 7 (BMP7))

Autre désignation: BMP-7 (BMP7 Produits)

Synonymes: bmp-7 Kit ELISA, bmp7 Kit ELISA, op-1 Kit ELISA, BMP7 Kit ELISA, zgc:153025 Kit ELISA, OP-1 Kit ELISA, OP1 Kit ELISA, BMP-7 Kit ELISA, fb83f09 Kit ELISA, wu:fb83f09 Kit ELISA, bone morphogenetic protein 7 Kit ELISA, bone morphogenetic protein 7, gene 1 L homeolog Kit ELISA, bone morphogenetic protein 7b Kit ELISA, bone morphogenetic protein 7, gene 2 L homeolog Kit ELISA, bone morphogenetic protein 7a Kit ELISA, BMP7 Kit ELISA, bmp7.1.L Kit ELISA, LOC100136282 Kit ELISA, bmp7b Kit ELISA, Bmp7 Kit ELISA, LOC100008826 Kit ELISA, bmp7.2.L Kit ELISA, bmp7a Kit ELISA

Sujet: Gene Names: Bmp7 Bmp-7 Op1
Protein names: Bone morphogenetic protein 7 (BMP-7) (Osteogenic protein 1) (OP-1)

ID gène: 12162

UniProt: P23359

Pathways: Steroid Hormone Mediated Signaling Pathway, Stem Cell Maintenance