MAPT Kit ELISA

N° du produit ABIN2703476

Kit ELISA MAPT Humain, Colorimetric test pour la quantification de Humain MAPT.

Aperçu rapide pour MAPT Kit ELISA (ABIN2703476)

Antigène: MAPT (Microtubule-Associated Protein tau (MAPT))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 100 pg/mL - 25000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Détail du produit MAPT Kit ELISA

Seuil minimal de détection: 100 pg/mL

Fonction: Human MAPT (Tau) ELISA Kit for cell culture supernatants, EDTA and/or Citrate treated plasma, and serum samples. Heparin is not recommended.

Analytical Method: Quantitative

Specificité: This ELISA antibody pair detects human Tau. Other species not determined.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Suggested Plasma Dilution: 2 fold

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Préparation de l'échantillon:

Collect plasma using EDTA and/or Citrate as the anticoagulant. Heparin is not recommended.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: MAPT (Microtubule-Associated Protein tau (MAPT))

Autre désignation: tau

Sujet:

Gene Symbols: MAPT, MAPTL, MTBT1, TAU

Protein Name (Synonyms): Microtubule-associated protein tau (Neurofibrillary tangle protein) (Paired helical filament-tau) (PHF-tau)

ID gène: 4137

UniProt: P10636

Pathways: Signalisation MAPK, Dynamique des Microtubules, M Phase, Regulation of Cell Size