BDNF Kit ELISA

N° du produit ABIN2708077

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain BDNF. Il a été cité dans 1 publication.

Aperçu rapide pour BDNF Kit ELISA (ABIN2708077)

Antigène: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156 pg/mL - 10000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate

Détail du produit BDNF Kit ELISA

Seuil minimal de détection: 156 pg/mL

Fonction: Sandwich ELISA for quantitative detection of Human BDNF concentrations.

Analytical Method: Quantitative

Sensibilité: 75 pg/mL

Ingrédients:

• Coated 96-well Plate
• Standard
• Biotin-Labeled Detection Antibody (100X)
• Streptavidin-HRP (100X)
• Standard/Sample Diluent
• Detection Antibody Diluent
• Streptavidin-HRP Diluent
• Wash Buffer (20X)
• TMB Substrate Solution
• Stop Solution
• Plate Adhesive Strips
• Technical Manual

Matériel non inclus:

1. Microplate reader capable of measuring absorbance at 450 nm.
2. Adjustable pipettes and pipette tips to deliver 2 µl to 1 ml volumes.
3. Adjustable 1-25 ml pipettes for reagent preparation.
4. 100 ml and 1 liter graduated cylinders.
5. Absorbent paper.
6. Distilled or deionized water.
7. Computer and software for ELISA data analysis.
8. Tubes to prepare standard or sample dilutions.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

• Prepare all reagents, samples and standards
• Add 100 µL Standard or Sample
• Wash plate 3 times with Wash Buffer Working Solution
• Add 100 µL Biotin-Labeled Detection Antibody Working Solution
• Wash plate 3 times with Wash Buffer Working Solution
• Add 100 µL Steptavidin-HRP Working Solution
• Wash plate 5 times with Wash Buffer Working Solution
• Add 100 µL TMB Substrat Solution
• Add 100 µL Stop Solution
• Read the plate at 450 nm

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid freeze/thaw cycles

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped and store at 4°C for 6 months, store at -20°C for one year.

Date de péremption: 6-12 months

Références pour BDNF Kit ELISA (ABIN2708077)

Zierold, Buschmann, Gachkar, Bochenek, Velmeden, Hobohm, Vahl, Schäfer: "Brain-Derived Neurotrophic Factor Expression and Signaling in Different Perivascular Adipose Tissue Depots of Patients With Coronary Artery Disease." dans: Journal of the American Heart Association, Vol. 10, Issue 6, pp. e018322, (2021) (PubMed).

Détail du antigène

Antigène: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Autre désignation: BDNF

Sujet: Alternative Names: Brain-derived neurotrophic factor, BDNF, Abrineurin

ID gène: 627

UniProt: P23560

Pathways: Signalisation RTK, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity