DKK1 Kit ELISA
Aperçu rapide pour DKK1 Kit ELISA (ABIN2708497)
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Voir toutes DKK1 Kits ELISAReactivité
Méthode de détection
Type de méthode
Gamme de detection
Application
Type d'échantillon
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Seuil minimal de détection
- 312 pg/mL
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Fonction
- Sandwich ELISA for quantitative detection of Mouse DKK1 concentrations.
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Analytical Method
- Quantitative
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Sensibilité
- 125 pg/mL
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Ingrédients
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- Coated 96-well Plate
- Standard
- Biotin-Labeled Detection Antibody (100X)
- Streptavidin-HRP (100X)
- Standard/Sample Diluent
- Detection Antibody Diluent
- Streptavidin-HRP Diluent
- Wash Buffer (20X)
- TMB Substrate Solution
- Stop Solution
- Plate Adhesive Strips
- Technical Manual
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Matériel non inclus
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- Microplate reader capable of measuring absorbance at 450 nm.
- Adjustable pipettes and pipette tips to deliver 2 µl to 1 ml volumes.
- Adjustable 1-25 ml pipettes for reagent preparation.
- 100 ml and 1 liter graduated cylinders.
- Absorbent paper.
- Distilled or deionized water.
- Computer and software for ELISA data analysis.
- Tubes to prepare standard or sample dilutions.
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Dickkopf Homolog 1 (DKK1) ELISA Kit
DKK1 Reactivité: Souris Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Dickkopf Homolog 1 (DKK1) ELISA KitDKK1 Reactivité: Humain Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Dickkopf Homolog 1 (DKK1) ELISA KitDKK1 Reactivité: Rat Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Dickkopf Homolog 1 (DKK1) ELISA KitDKK1 Reactivité: Souris AA 30-272 Colorimetric Sandwich ELISA 46.9-3000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Dickkopf Homolog 1 (DKK1) ELISA KitDKK1 Reactivité: Humain Colorimetric Sandwich ELISA 63-4000 pg/mL Cell Lysate, Plasma, Serum
Dickkopf Homolog 1 (DKK1) ELISA KitDKK1 Reactivité: Humain AA 32-266 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Dickkopf Homolog 1 (DKK1) ELISA KitDKK1 Reactivité: Rat AA 23-270 Colorimetric Sandwich ELISA 46.9-3000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Dickkopf Homolog 1 (DKK1) ELISA KitDKK1 Reactivité: Humain Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Plasma, Serum
Dickkopf Homolog 1 (DKK1) ELISA KitDKK1 Reactivité: Souris Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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Indications d'application
- Optimal working dilution should be determined by the investigator.
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Volume d'échantillon
- 100 μL
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Plaque
- Pre-coated
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Protocole
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- Prepare all reagents, samples and standards
- Add 100 µL Standard or Sample
- Wash plate 3 times with Wash Buffer Working Solution
- Add 100 µL Biotin-Labeled Detection Antibody Working Solution
- Wash plate 3 times with Wash Buffer Working Solution
- Add 100 µL Steptavidin-HRP Working Solution
- Wash plate 5 times with Wash Buffer Working Solution
- Add 100 µL TMB Substrat Solution
- Add 100 µL Stop Solution
- Read the plate at 450 nm
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Restrictions
- For Research Use only
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Conseil sur la manipulation
- Avoid freeze/thaw cycles
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Shipped and store at 4°C for 6 months, store at -20°C for one year.
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Date de péremption
- 6-12 months
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- DKK1 (Dickkopf Homolog 1 (DKK1))
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Autre désignation
- DKK1
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Sujet
- Alternative Names: Dickkopf-related protein 1, Dickkopf-1, Dkk-1, hDkk-1, SK
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ID gène
- 13380
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UniProt
- O54908
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Pathways
- Signalisation WNT, Regulation of Muscle Cell Differentiation, Positive Regulation of fat Cell Differentiation
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