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SMAD2 Kit ELISA

SMAD2 Reactivité: Humain, Rat, Souris pSer255 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
N° du produit ABIN2747901
  • Antigène Voir toutes SMAD2 Kits ELISA
    SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))
    Épitope
    pSer245, pSer250, pSer255, total
    Reactivité
    • 7
    • 6
    • 5
    • 1
    Humain, Rat, Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human/Mouse/Rat Phospho-SMAD2 (SER245/250/255) and Total SMAD2 Kit. This assay semi-quantitatively measures phophorylated SMAD2 (Ser245/250/255) and Total SMAD2 in lysate samples.
    Type d'échantillon
    Cell Lysate, Tissue Lysate
    Analytical Method
    Semi-Quantitative
    Specificité
    The antibody pair provided in this kit recognizes human, mouse, rat SMAD2 phosphorylated at site Serine-245/250/255 and Total SMAD2.
    Attributs du produit
    • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • Anti-Pan Antibody
    • HRP-Conjugated Secondary Antibody
    • Streptavidin-Conjugated HRP
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Matériel non inclus
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
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  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Procédure de l'essai

    Prepare all reagents and samples as instructed in the manual.
    Add 100 μL of sample or positive control to each well.
    Incubate 2.5 h at RT or O/N at 4 °C.
    Add 100 μL of prepared primary antibody to each well.
    Incubate 1 h at RT.
    Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    Incubate 1 h at RT.
    Add 100 μL of TMB One-Step Substrate Reagent to each well.
    Incubate 30 min at RT.
    Add 50 μL of Stop Solution to each well.
    Read at 450 nm immediately.

    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Date de péremption
    6 months
  • Antigène Voir toutes SMAD2 Kits ELISA
    SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))
    Autre désignation
    SMAD2 (SMAD2 Produits)
    Synonymes
    JV18 Kit ELISA, JV18-1 Kit ELISA, MADH2 Kit ELISA, MADR2 Kit ELISA, hMAD-2 Kit ELISA, hSMAD2 Kit ELISA, jv18 Kit ELISA, Xmad2 Kit ELISA, madh2 Kit ELISA, madr2 Kit ELISA, XSmad2 Kit ELISA, hmad-2 Kit ELISA, hsmad2 Kit ELISA, jv18-1 Kit ELISA, smad-2 Kit ELISA, fj43c06 Kit ELISA, wu:fj43c06 Kit ELISA, 7120426M23Rik Kit ELISA, Madh2 Kit ELISA, Madr2 Kit ELISA, Smad-2 Kit ELISA, mMad2 Kit ELISA, MAD homolog 2 Kit ELISA, Mothers against DPP homolog 2 Kit ELISA, SMAD 2 Kit ELISA, Smad2 Kit ELISA, SMAD family member 2 Kit ELISA, SMAD family member 2-Z Kit ELISA, SMAD family member 2 S homeolog Kit ELISA, SMAD2 Kit ELISA, SMAD2Z Kit ELISA, smad2 Kit ELISA, smad2.S Kit ELISA, Smad2 Kit ELISA
    Sujet
    Mothers against decapentaplegic homolog 2 (SMAD2) phosphorylated at Serine-245/250/255 and Total SMAD2
    ID gène
    4087
    UniProt
    Q15796
    Pathways
    Cycle Cellulaire, Hormone Transport, Chromatin Binding, Protein targeting to Nucleus
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