ADAM9 Kit ELISA

N° du produit ABIN2747928

Détail du produit ADAM9 Kit ELISA

Antigène: ADAM9 (ADAM Metallopeptidase Domain 9 (ADAM9))

Reactivité: Macaque rhésus

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Rhesus Macaque ADAM metallopeptidase domain 9 (ADAM9) ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: The antibody pair provided in this kit recognizes Rhesus Macaque ADAM metallopeptidase domain 9 (ADAM9)

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Procédure de l'essai:

Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: ADAM9 (ADAM Metallopeptidase Domain 9 (ADAM9))

Autre désignation: ADAM9 (ADAM9 Produits)

Synonymes: CORD9 Kit ELISA, MCMP Kit ELISA, MDC9 Kit ELISA, Mltng Kit ELISA, AU020942 Kit ELISA, mKIAA0021 Kit ELISA, adam9-A Kit ELISA, cord9 Kit ELISA, mcmp Kit ELISA, mltng Kit ELISA, Adam9 Kit ELISA, zgc:101824 Kit ELISA, ADAM9 Kit ELISA, ADAM metallopeptidase domain 9 Kit ELISA, a disintegrin and metallopeptidase domain 9 (meltrin gamma) Kit ELISA, ADAM metallopeptidase domain 9 L homeolog Kit ELISA, ADAM9 Kit ELISA, Adam9 Kit ELISA, adam9.L Kit ELISA, adam9 Kit ELISA

Sujet: ADAM metallopeptidase domain 9 (ADAM9)

UniProt: F6SG12

Pathways: Cellular Response to Molecule of Bacterial Origin, SARS-CoV-2 Protein Interactome