DKK1 Kit ELISA

N° du produit ABIN2748048

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Rat DKK1.

Aperçu rapide pour DKK1 Kit ELISA (ABIN2748048)

Antigène: DKK1 (Dickkopf Homolog 1 (DKK1))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 33 pg/mL - 8000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Détail du produit DKK1 Kit ELISA

Seuil minimal de détection: 33 pg/mL

Fonction: Rat DKK-1 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Analytical Method: Quantitative

Specificité: This ELISA antibody pair detects Rat DKK-1 and Mouse DKK-1. Other species not determined.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate< div class='ty-product-feature'>< div class='ty-product-feature__label'>Other Materials Required< div class='ty-product-feature__value'>
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μl to 1 μl volumes
  • Adjustable 1-25 μl pipettes for reagent preparation
  • 100 μl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
  ,
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate< div class='ty-product-feature'>< div class='ty-product-feature__label'>Other Materials Required< div class='ty-product-feature__value'>
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μl to 1 μl volumes
    • Adjustable 1-25 μl pipettes for reagent preparation
    • 100 μl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Suggested Plasma Dilution: 2 fold

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4^°C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Procédure de l'essai:

Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: May be stored for up to 6 months at 2^° to 8^°C from the date of shipment. Standard (recombinant protein) should be stored at -20^°C or -80^°C (recommended at -80^°C) after reconstitution. Opened Microplate Wells or reagents may be store for up to 1 month at 2^° to 8^°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: the kit can be used within one year if the whole kit is stored at -20^°C.

Date de péremption: 6 months

Détail du antigène

Antigène: DKK1 (Dickkopf Homolog 1 (DKK1))

Autre désignation: Dkk-1

Sujet:

Gene Symbols: DKK1, DKK1_PREDICTED, LOC100912050, NEWGENE_1307313, RCG_48380

Protein Name (Synonyms): Dickkopf WNT-signaling pathway inhibitor 1 (Dickkopf homolog 1 (Xenopus laevis)) (Dickkopf-like protein 1)

ID gène: 293897108348170, 293897

UniProt: D3Z9J1

Pathways: Signalisation WNT, Regulation of Muscle Cell Differentiation, Positive Regulation of fat Cell Differentiation