GDNF Kit ELISA

N° du produit ABIN2748140

Détail du produit GDNF Kit ELISA

Antigène: GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 60-15.000 pg/mL

Seuil minimal de détection: 60 pg/mL

Application: ELISA

Fonction: Rat GDNF ELISA Kit for cell culture supernatants, plasma, and serum samples.

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificité: This ELISA antibody pair detects rat and human GDNF. Other species not determined.

Sensibilité: 60 pg/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples2 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Procédure de l'essai:

Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))

Autre désignation: GDNF (GDNF Produits)

Synonymes: gdnf Kit ELISA, GDNF Kit ELISA, AI385739 Kit ELISA, gdnfa Kit ELISA, hfb1-gdnf Kit ELISA, ATF1 Kit ELISA, ATF2 Kit ELISA, HFB1-GDNF Kit ELISA, HSCR3 Kit ELISA, glial cell derived neurotrophic factor Kit ELISA, glial cell derived neurotrophic factor a Kit ELISA, glial cell line derived neurotrophic factor Kit ELISA, glial cell derived neurotrophic factor L homeolog Kit ELISA, Gdnf Kit ELISA, gdnfa Kit ELISA, GDNF Kit ELISA, gdnf.L Kit ELISA

Sujet: Glial cell line-derived neurotrophic factor (Astrocyte-derived trophic factor) (ATF)

ID gène: 25453

UniProt: Q07731

Pathways: Signalisation RTK, Synaptic Membrane, Tube Formation, Autophagy, Smooth Muscle Cell Migration