IGF2 Kit ELISA

N° du produit ABIN2748182

Détail du produit IGF2 Kit ELISA

Antigène: IGF2 (Insulin-Like Growth Factor 2 (IGF2))

Reactivité: Macaque rhésus

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.2-50 ng/mL

Seuil minimal de détection: 0.2 ng/mL

Application: ELISA

Fonction: Rhesus Monkey IGF-2 ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificité: The antibody pair provided in this kit recognizes Rhesus Macaque Insulin-like Growth Factor 2 (IGF2)

Sensibilité: 24 pg/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples2 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: IGF2 (Insulin-Like Growth Factor 2 (IGF2))

Autre désignation: IGF-2 (IGF2 Produits)

Synonymes: C11orf43 Kit ELISA, IGF-II Kit ELISA, PP9974 Kit ELISA, LOC100136550 Kit ELISA, igf2 Kit ELISA, igf2b Kit ELISA, wu:fc26e03 Kit ELISA, zgc:91781 Kit ELISA, igf-2 Kit ELISA, insigf Kit ELISA, pp9974 Kit ELISA, AL033362 Kit ELISA, Igf-2 Kit ELISA, Igf-II Kit ELISA, M6pr Kit ELISA, Mpr Kit ELISA, Peg2 Kit ELISA, IGFII Kit ELISA, RNIGF2 Kit ELISA, IGF-II-B Kit ELISA, igf2-a Kit ELISA, igf2-b Kit ELISA, insulin like growth factor 2 Kit ELISA, insulin-like growth factor II Kit ELISA, insulin-like growth factor 2a Kit ELISA, insulin like growth factor 2 S homeolog Kit ELISA, insulin-like growth factor 2 Kit ELISA, insulin-like growth factor 2 (somatomedin A) Kit ELISA, insulin like growth factor 2 L homeolog Kit ELISA, IGF2 Kit ELISA, LOC100136550 Kit ELISA, igf2a Kit ELISA, igf2.S Kit ELISA, Igf2 Kit ELISA, igf2.L Kit ELISA

Sujet: Gene Names: IFNG
Protein names: Interferon gamma (IFN-gamma)

UniProt: G7NBL8

Pathways: Hormone Activity, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Activated T Cell Proliferation