Neuropilin 1 Kit ELISA

N° du produit ABIN2748424

Le kit ELISA Neuropilin 1 (ABIN2748424) Rat est un kit ELISA Colorimetric conçu pour quantifier Rat Neuropilin 1.

Aperçu rapide pour Neuropilin 1 Kit ELISA (ABIN2748424)

Antigène: Neuropilin 1 (NRP1)

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.03 ng/mL - 20 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Détail du produit Neuropilin 1 Kit ELISA

Seuil minimal de détection: 0.03 ng/mL

Fonction: The Rat Neuropilin-1 ELISA Kit is a pre-coated sandwich-based assay kit designed to quantitatively measure Neuropilin-1 (NRP1) levels in rat cell culture media, plasma, and serum.

Analytical Method: Quantitative

Specificité: This ELISA antibody pair detects rat NRP1. Other species not determined.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• Wash Buffer
• TMB One-Step Substrate Reagent
• Stop Solution
• Assay Diluent(s)
• Adhesive Sealing Film

Matériel non inclus:

• Microplate reader capable of measuring absorbance at 450 nm
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Absorbent paper
• Distilled or deionized water
• Log-log graph paper or computer and software for ELISA data analysis
• Tubes to prepare standard or sample dilutions
• Plastic wrap

Information d'application

Indications d'application: Suggested Plasma Dilution: 2 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL standard or sample to each well. Incubate 2.5 hours at room temperature.
3. Add 100 μL prepared biotin antibody to each well. Incubate 1 hour at room temperature.
4. Add 100 μL prepared Streptavidin solution to each well. Incubate 45 minutes at room temperature.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately

Procédure de l'essai:

Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: Neuropilin 1 (NRP1)

Autre désignation: Neuropilin-1

Sujet:

Gene Symbols: NRP1

Protein Name (Synonyms): Neuropilin-1 (Vascular endothelial cell growth factor 165 receptor) (CD antigen CD304)

ID gène: 246331

UniProt: Q9QWJ9

Pathways: Regulation of Cell Size, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals