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CCL5 Kit ELISA

CCL5 Reactivité: Porc Colorimetric Sandwich ELISA 46.9-3000 pg/mL Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN2748589
  • Antigène Voir toutes CCL5 Kits ELISA
    CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))
    Reactivité
    • 6
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Porc
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    46.9-3000 pg/mL
    Seuil minimal de détection
    46.9 pg/mL
    Application
    ELISA
    Fonction
    Porcine RANTES (CCL5) ELISA Kit for serum, plasma, and cell culture supernatant samples.
    Type d'échantillon
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificité
    Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: porcine Angiopoietin-1, IL-17F, MIF, Osteoprotegerin (TNFRSF11B), PDGF-BB, TGF alpha, TIMP-1, TIMP-2, VEGF-A
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Indications d'application
    Recommended Dilution for serum and plasma samples2 fold
    Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Procédure de l'essai

    Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes CCL5 Kits ELISA
    CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))
    Autre désignation
    RANTES (CCL5 Produits)
    Synonymes
    Rantes Kit ELISA, Scya5 Kit ELISA, D17S136E Kit ELISA, RANTES Kit ELISA, SCYA5 Kit ELISA, SIS-delta Kit ELISA, SISd Kit ELISA, TCP228 Kit ELISA, eoCP Kit ELISA, MuRantes Kit ELISA, C-C motif chemokine 5-like Kit ELISA, C-C motif chemokine ligand 5 Kit ELISA, chemokine (C-C motif) ligand 5 Kit ELISA, LOC100230935 Kit ELISA, CCL5 Kit ELISA, Ccl5 Kit ELISA
    Sujet
    RANTES (CCL5)
    UniProt
    Q29288
    Pathways
    Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration
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