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FRZB Kit ELISA

FRZB Reactivité: Souris Colorimetric Sandwich ELISA Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN2748637
  • Antigène Voir toutes FRZB Kits ELISA
    FRZB (Frizzled-Related Protein (FRZB))
    Reactivité
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Mouse sFRP-3 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
    Type d'échantillon
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair recognizes Mouse sFRP-3.
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Procédure de l'essai

    Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes FRZB Kits ELISA
    FRZB (Frizzled-Related Protein (FRZB))
    Autre désignation
    sFRP-3 (FRZB Produits)
    Synonymes
    Frp Kit ELISA, Sfrp3 Kit ELISA, frezzled Kit ELISA, fritz Kit ELISA, frzb-1 Kit ELISA, FRE Kit ELISA, FRITZ Kit ELISA, FRP-3 Kit ELISA, FRZB-1 Kit ELISA, FRZB-PEN Kit ELISA, FRZB1 Kit ELISA, FZRB Kit ELISA, OS1 Kit ELISA, SFRP3 Kit ELISA, SRFP3 Kit ELISA, hFIZ Kit ELISA, Frzb-1 Kit ELISA, frizzled-related protein Kit ELISA, Frzb Kit ELISA, FRZB Kit ELISA
    Sujet
    Secreted Frizzled Related Protein 3 (FRZB)
    ID gène
    20378
    UniProt
    P97401
    Pathways
    Signalisation WNT, Positive Regulation of fat Cell Differentiation
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