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ANGPTL3 Kit ELISA

ANGPTL3 Reactivité: Humain AA 17-460 Colorimetric Sandwich ELISA 312-20.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
N° du produit ABIN2859241
  • Antigène Voir toutes ANGPTL3 Kits ELISA
    ANGPTL3 (Angiopoietin-Like 3 (ANGPTL3))
    Épitope
    AA 17-460
    Reactivité
    • 5
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    312-20.000 pg/mL
    Seuil minimal de détection
    312 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human ANGPTL3
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: sf21
    Immunogen sequence: S17-E460
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <10pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: sf21
    Immunogen sequence: S17-E460
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  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Commentaires

    Sequence similarities: Contains 1 fibrinogen C-terminal domain.

    Tissue Specificity: Expressed principally in liver. Weakly expressed in kidney. .

    Plaque
    Pre-coated
    Protocole
    human ANGPTL3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ANGPTL3 has been precoated onto 96-well plates. Standards(sf21, S17-E460) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ANGPTL3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ANGPTL3 amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 20,000pg/mL, 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human ANGPTL3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human ANGPTL3 standard solution and each sample be measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(ng/ml): 3.2, Standard deviation: 0.144, CV(%): 4.5
    • Sample 2: n=16, Mean(ng/ml): 5.8, Standard deviation: 0.273, CV(%): 4.7
    • Sample 3: n=16, Mean(ng/ml): 10.3, Standard deviation: 0.299, CV(%): 2.9,
    • Sample 1: n=24, Mean(ng/ml): 3.5, Standard deviation: 0.238, CV(%): 6.8
    • Sample 2: n=24, Mean(ng/ml): 6.2, Standard deviation: 0.459, CV(%): 7.4
    • Sample 3: n=24, Mean(ng/ml): 12.1, Standard deviation: 0.762, CV(%): 6.3
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Antigène Voir toutes ANGPTL3 Kits ELISA
    ANGPTL3 (Angiopoietin-Like 3 (ANGPTL3))
    Autre désignation
    ANGPTL3 (ANGPTL3 Produits)
    Synonymes
    fb60e11 Kit ELISA, fb66h02 Kit ELISA, wu:fb60e11 Kit ELISA, wu:fb66h02 Kit ELISA, zgc:111943 Kit ELISA, ANGPTL3 Kit ELISA, LOC100230785 Kit ELISA, ANG-5 Kit ELISA, ANGPT5 Kit ELISA, ANL3 Kit ELISA, FHBL2 Kit ELISA, hypl Kit ELISA, angiopoietin-like 3 Kit ELISA, angiopoietin like 3 Kit ELISA, angptl3 Kit ELISA, ANGPTL3 Kit ELISA, Angptl3 Kit ELISA
    Sujet

    Background: ANGPTL3, also known as Angiopoietin-like 3 or HYPLl, is a protein that in humans is encoded by the ANGPTL3 gene. It is mapped to 1p31.3. The protein encoded by this gene is a member of the angiopoietin-like family of secreted factors. It is expressed predominantly in the liver, and has the characteristic structure of angiopoietins, consisting of a signal peptide, N-terminal coiled-coil domain, and the C-terminal fibrinogen (FBN)-like domain. ANGPTL3 also acts as dual inhibitor of lipoprotein lipase (LPL) and endothelial lipase (EL), and increases plasma triglyceride and HDL cholesterol in rodents. It can inhibit endothelial lipase to catalyze HDL-phospholipid and increase HDL-PL levels.

    Synonyms: Angiopoietin-related protein 3,Angiopoietin-5,ANG-5,Angiopoietin-like protein 3,ANGPTL3,ANGPT5,UNQ153/PRO179,

    Full Gene Name: Angiopoietin-related protein 3

    Cellular Localisation: Secreted.
    ID gène
    27329
    UniProt
    Q9Y5C1
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