ANGPTL3 Kit ELISA

N° du produit ABIN6719722

Détail du produit ANGPTL3 Kit ELISA

Antigène: ANGPTL3 (Angiopoietin-Like 3 (ANGPTL3))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 312 pg/mL - 20000 pg/mL

Seuil minimal de détection: 312 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: ANG 5, ANG-5, ANG5, Angiopoietin 5, Angiopoietin like 3, Angiopoietin related protein 3, Angiopoietin-5, Angiopoietin-like protein 3, Angiopoietin-related protein 3, ANGL3_HUMAN, ANGPT5, ANGPTL3, ANL3, FHBL2, OTTHUMP00000010719, UNQ153/PRO179

Background: ANGPTL3, also known as Angiopoietin-like 3 or HYPLl, is a protein that in humans is encoded by the ANGPTL3 gene. It is mapped to 1p31.3. The protein encoded by this gene is a member of the angiopoietin-like family of secreted factors. It is expressed predominantly in the liver, and has the characteristic structure of angiopoietins, consisting of a signal peptide, N-terminal coiled-coil domain, and the C-terminal fibrinogen (FBN)-like domain. ANGPTL3 also acts as dual inhibitor of lipoprotein lipase (LPL) and endothelial lipase (EL), and increases plasma triglyceride and HDL cholesterol in rodents. It can inhibit endothelial lipase to catalyze HDL-phospholipid and increase HDL-PL levels.

Gene Name: ANGPTL3

Production: Natural and recombinant human ANGPTL3. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: sf21, Immunogen sequence: S17-E460

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 20,000pg/mL, 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human ANGPTL3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human ANGPTL3 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 312pg/mL-20,000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: ANGPTL3 (Angiopoietin-Like 3 (ANGPTL3))

Autre désignation: ANGPTL3 (ANGPTL3 Produits)

Synonymes: fb60e11 Kit ELISA, fb66h02 Kit ELISA, wu:fb60e11 Kit ELISA, wu:fb66h02 Kit ELISA, zgc:111943 Kit ELISA, ANGPTL3 Kit ELISA, LOC100230785 Kit ELISA, ANG-5 Kit ELISA, ANGPT5 Kit ELISA, ANL3 Kit ELISA, FHBL2 Kit ELISA, hypl Kit ELISA, angiopoietin-like 3 Kit ELISA, angiopoietin like 3 Kit ELISA, angptl3 Kit ELISA, ANGPTL3 Kit ELISA, Angptl3 Kit ELISA

ID gène: 27329

NCBI Accession: NP_055310

UniProt: Q9Y5C1