Complement Factor H Kit ELISA

N° du produit ABIN2859251

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Humain Complement Factor H.

Aperçu rapide pour Complement Factor H Kit ELISA (ABIN2859251)

Antigène: Complement Factor H (CFH)

Épitope: AA 860-1231

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.56-100 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Urine

Détail du produit Complement Factor H Kit ELISA

Seuil minimal de détection: 1.56 ng/mL

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Complement H/CFH

Marque: PicoKine™

Analytical Method: Quantitative

Specificité: NSO, S860-R1231

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <50pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: S860-R1231

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Tissue Specificity: Expressed by the liver and secreted in plasma.

Plaque: Pre-coated

Protocole: human CFH ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CFH has been precoated onto 96-well plates. Standards (NSO, S860-R1231) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CFH is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CFH amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 100 ng/mL, 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL human CFH standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CFH standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(ng/ml): 2.32, Standard deviation: 0.12, CV(%): 5.2
• Sample 2: n=16, Mean(ng/ml): 8.15, Standard deviation: 0.47, CV(%): 5.8
• Sample 3: n=16, Mean(ng/ml): 37.66, Standard deviation: 2.37, CV(%): 6.3,
• Sample 1: n=24, Mean(ng/ml): 2.01, Standard deviation: 0.13, CV(%): 6.9
• Sample 2: n=24, Mean(ng/ml): 9.00, Standard deviation: 0.56, CV(%): 6.3
• Sample 3: n=24, Mean(ng/ml): 45.39, Standard deviation: 3.22, CV(%): 7.1

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: Complement Factor H (CFH)

Autre désignation: CFH

Sujet:

Protein Function: Factor H functions as a cofactor in the inactivation of C3b by factor I and also increases the rate of dissociation of the C3bBb complex (C3 convertase) and the (C3b)NBB complex (C5 convertase) in the alternative complement pathway.

Background: Complement factor H (CFH), originally known as beta-1H globulin, is a serum glycoprotein that regulates the function of the alternativecomplement pathway in fluid phase and on cellular surfaces. It binds to C3b, accelerates the decay of the alternative pathway convertase C3bBb, and also acts as a cofactor for complement factor I, another C3b inhibitor. The CFH gene is located on chromosome 1q32-q32.1 within a cluster of genes encoding the regulatory complement components of the activation of C3 (RCA for 'regulators of complement activation'). This gene cluster includes decay-accelerating factor (DAF), C4-binding protein (C4BPA and C4BPB), and the factor H-related genes CFHR1, CFHR2, CFHR3, CFHR4, and CFHR5, among others. The gene family has arisen by multiple duplication events.

Synonyms: Complement factor H,H factor 1,CFH,HF, HF1, HF2,

Full Gene Name: Complement factor H

Cellular Localisation: Secreted.

ID gène: 3075

UniProt: P08603

Pathways: Système du Complément, Cellular Response to Molecule of Bacterial Origin