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CXCL2 Kit ELISA

CXCL2 Reactivité: Rat AA 32-100 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
N° du produit ABIN2859287
  • Antigène Voir toutes CXCL2 Kits ELISA
    CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))
    Épitope
    AA 32-100
    Reactivité
    • 7
    • 6
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Rat
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    15.6-1000 pg/mL
    Seuil minimal de détection
    15.6 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat CXCL2/MIP-2
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificité
    E.coli, S32-N100
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <10pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: E.coli
    Immunogen sequence: S32-N100
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  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Commentaires

    Tissue Specificity: At least expressed in the lung and trachea.

    Plaque
    Pre-coated
    Protocole
    rat MIP-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MIP-2 has been precoated onto 96-well plates. Standards (E.coli, S32-N100) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MIP-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat MIP-2 amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL rat MIP-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat MIP-2 standard solution and each sample be measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(pg/ml): 113, Standard deviation: 4.97, CV(%): 4.4
    • Sample 2: n=16, Mean(pg/ml): 355, Standard deviation: 18.46, CV(%): 5.2
    • Sample 3: n=16, Mean(pg/ml): 627, Standard deviation: 25.73, CV(%): 5.7,
    • Sample 1: n=24, Mean(pg/ml): 134, Standard deviation: 6.96, CV(%): 5.2
    • Sample 2: n=24, Mean(pg/ml): 387, Standard deviation: 21.28, CV(%): 5.5
    • Sample 3: n=24, Mean(pg/ml): 653, Standard deviation: 39.18, CV(%): 6.0
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Zhu, He, Liu, Zhang, Yang, Liu, Wang: "Alpha 1-antitrypsin ameliorates ventilator-induced lung injury in rats by inhibiting inflammatory responses and apoptosis." dans: Experimental biology and medicine (Maywood, N.J.), Vol. 243, Issue 1, pp. 87-95, (2018) (PubMed).

    Mei, Wang, Li, Liu, Lu, Li, Zhang, Tian: "Dusuqing granules (DSQ) suppress inflammation in Klebsiella pneumonia rat via NF-κB/MAPK signaling." dans: BMC complementary and alternative medicine, Vol. 17, Issue 1, pp. 216, (2017) (PubMed).

  • Antigène Voir toutes CXCL2 Kits ELISA
    CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))
    Autre désignation
    CXCL2 (CXCL2 Produits)
    Synonymes
    CINC-2a Kit ELISA, GRO2 Kit ELISA, GROb Kit ELISA, MGSA-b Kit ELISA, MIP-2a Kit ELISA, MIP2 Kit ELISA, MIP2A Kit ELISA, SCYB2 Kit ELISA, GRO3 Kit ELISA, Gro2 Kit ELISA, MIP-2 Kit ELISA, Mgsa-b Kit ELISA, Mip2 Kit ELISA, Scyb Kit ELISA, Scyb2 Kit ELISA, Mip-2 Kit ELISA, GROB Kit ELISA, MIP-2alpha Kit ELISA, C-X-C motif chemokine ligand 2 Kit ELISA, chemokine (C-X-C motif) ligand 2 Kit ELISA, CXCL2 Kit ELISA, Cxcl2 Kit ELISA
    Sujet

    Protein Function: Chemotactic for human polymorphonuclear leukocytes but does not induce chemokinesis or an oxidative burst. Contributes to neutrophil activation during inflammation.

    Background: MIP is a member of the aquaporin family of membrane-bound water channels. MIP family proteins are thought to contain 6 TM domains. Sequence analysis suggests that the proteins may have arisen through tandem, intragenic duplication from an ancestral protein that contained 3 TM domains. Major intrinsic protein (MIP, also called MP26) is the predominant fiber cell membrane protein of the ocular lens. The major intrinsic protein (MIP) of the vertebrate eye lens is the first identified member of a sequence-related family of cell-membrane proteins that appears to have evolved by gene duplication. Several members of the MIP family transport water (aquaporins), glycerol and other small molecules in microbial, plant and animal cells.

    Synonyms: C-X-C motif chemokine 2,Cytokine-induced neutrophil chemoattractant 3,CINC-3,Macrophage inflammatory protein 2,MIP2,Cxcl2,Cinc3, Mip-2, Mip2, Scyb2,

    Full Gene Name: C-X-C motif chemokine 2

    Cellular Localisation: Secreted.
    ID gène
    114105
    UniProt
    P30348
    Pathways
    Cellular Response to Molecule of Bacterial Origin
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