CXCL2 Kit ELISA

N° du produit ABIN921067

Kit ELISA CXCL2 Souris, Colorimetric test pour la quantification de Souris CXCL2 et a été mentionné dans 3+ publications.

Aperçu rapide pour CXCL2 Kit ELISA (ABIN921067)

Antigène: CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))

Épitope: AA 28-100

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6 pg/mL - 1000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Détail du produit CXCL2 Kit ELISA

Seuil minimal de détection: 15.6 pg/mL

Fonction: Sandwich Mouse MIP-2 ELISA Kit to quantitate Mouse Cxcl2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Analytical Method: Quantitative

Specificité: Expression system for standard: E.coli
Immunogen sequence: A28-N100

Capture antibody and Detection antibody: monoclonal antibody from rat, polyclonal antibody from goat

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 5 pg/mL

Ingrédients:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Précision du teste: Intra-Assay Precision (CV%): 4.6%, 7.8%, 4.1%
Inter-Assay Precision (CV%): 5%, 8.5%, 5.6%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Date de péremption: 12 months

Références pour CXCL2 Kit ELISA (ABIN921067)

Du, Chen, Wang, Sun: "Pathway analysis of global gene expression change in dendritic cells induced by the polysaccharide from the roots of Actinidia eriantha." dans: Journal of ethnopharmacology, Vol. 214, pp. 141-152, (2018) (PubMed).

Roux, Schaefers, Clark, Weatherholt, Renaud, Scott, LiPuma, Priebe, Gerard, Yoder-Himes: "A putative lateral flagella of the cystic fibrosis pathogen Burkholderia dolosa regulates swimming motility and host cytokine production." dans: PLoS ONE, Vol. 13, Issue 1, pp. e0189810, (2018) (PubMed).

Roux, Weatherholt, Clark, Gadjeva, Renaud, Scott, Skurnik, Priebe, Pier, Gerard, Yoder-Himes: "Immune Recognition of the Epidemic Cystic Fibrosis Pathogen Burkholderia dolosa." dans: Infection and immunity, Vol. 85, Issue 6, (2017) (PubMed).

Détail du antigène

Antigène: CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))

Autre désignation: CXCL2

Sujet:

Background: Macrophage Inflammatory Protein 2 (MIP2), also known as C-X-C motif chemokine 2 (CXCL2), is a small cytokine belonging to the CXC chemokine family. MIP2 forms homotetramers. MIP2 is produced by activated monocytes and neutrophils and expressed at sites of inflammation. MIP2 is chemotactic for polymorphonuclear leukocytes.

Gene Full Name: C-X-C motif chemokine ligand 2

ID gène: 20310

UniProt: P10889

Pathways: Cellular Response to Molecule of Bacterial Origin