MBL2 Kit ELISA

N° du produit ABIN2859302

Détail du produit MBL2 Kit ELISA

Antigène: MBL2 (Mannose-Binding Lectin (Protein C) 2, Soluble (MBL2))

Épitope: AA 21-248

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 312-20000 pg/mL

Seuil minimal de détection: 312 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human MBP-C

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: E21-I248

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: E21-I248

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Contains 1 C-type lectin domain.

Tissue Specificity: Plasma protein produced mainly in the liver. .

Plaque: Pre-coated

Protocole: human MBP-C ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MBP-C has been precoated onto 96-well plates. Standards(NSO, E21-I248) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MBP-C is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human MBP-C amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human MBP-C standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human MBP-C standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(ng/ml): 2.4, Standard deviation: 0.086, CV(%): 3.6
• Sample 2: n=16, Mean(ng/ml): 7.2, Standard deviation: 0.324, CV(%): 4.5
• Sample 3: n=16, Mean(ng/ml): 11.5, Standard deviation: 0.61, CV(%): 5.3,
• Sample 1: n=24, Mean(ng/ml): 3.2, Standard deviation: 0.157, CV(%): 4.9
• Sample 2: n=24, Mean(ng/ml): 6.9, Standard deviation: 0.393, CV(%): 5.7
• Sample 3: n=24, Mean(ng/ml): 10.7, Standard deviation: 0.738, CV(%): 6.9

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour MBL2 Kit ELISA (ABIN2859302)

Gomaa, Ali, Fattouh, Hamza, Badr: "MBL2 gene polymorphism rs1800450 and rheumatic fever with and without rheumatic heart disease: an Egyptian pilot study." dans: Pediatric rheumatology online journal, Vol. 16, Issue 1, pp. 24, (2018) (PubMed).

Détail du antigène

Antigène: MBL2 (Mannose-Binding Lectin (Protein C) 2, Soluble (MBL2))

Autre désignation: MBL2 (MBL2 Produits)

Sujet:

Protein Function: Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA. .

Background: MBL2, also called mannose-binding lectin (protein C) 2, soluble or Mannose-binding lectin (MBL) is a lectin that is instrumental in innate immunity. MBL2 is mapped to chromosome 10q11.2-q21. It belongs to the class of collectins in the C-type lectin superfamily, whose function appears to be pattern recognition in the first line of defense in the pre-immune host. MBL2 recognizes carbohydrate patterns, found on the surface of a large number of pathogenic micro-organisms, including bacteria, viruses, protozoa and fungi. Binding MBL2 to a micro-organism results in activation of the lectin pathway of the complement system. Another important function of MBL2 is that this molecule binds senescent and apoptotic cells and enhances engulfment of whole, intact apoptotic cells, as well as cell debris by phagocytes.

Synonyms: Mannose-binding protein C,MBP-C,Collectin-1,MBP1,Mannan-binding protein,Mannose-binding lectin,MBL2,COLEC1, MBL,

Full Gene Name: Mannose-binding protein C

Cellular Localisation: Secreted.

ID gène: 4153

UniProt: P11226

Pathways: Système du Complément, Positive Regulation of Immune Effector Process