HMOX1 Kit ELISA

N° du produit ABIN2859305

Détail du produit HMOX1 Kit ELISA

Antigène: HMOX1 (Heme Oxygenase (Decycling) 1 (HMOX1))

Épitope: AA 1-288

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 93.7-6000 pg/mL

Seuil minimal de détection: 93.7 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human HO-1/HMOX1

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: E.coli, M1-M288

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: E.coli
Immunogen sequence: M1-M288

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Tissue Specificity: Expressed at higher levels in renal cancer tissue than in normal tissue (at protein level). .

Plaque: Pre-coated

Protocole: human HO-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for HO-1 has been precoated onto 96-well plates. Standards(E.coli, M1-M288) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for HO-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human HO-1 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 6000pg/mL, 3000pg/mL, 1500pg/mL, 750pg/mL, 375pg/mL, 187.5pg/mL, 93.7pg/m human HO-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human HO-1 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 527, Standard deviation: 19, CV(%): 3.6
• Sample 2: n=16, Mean(pg/ml): 2085, Standard deviation: 91.74, CV(%): 4.4
• Sample 3: n=16, Mean(pg/ml): 3561, Standard deviation: 185.2, CV(%): 5.2,
• Sample 1: n=24, Mean(pg/ml): 686, Standard deviation: 31.6, CV(%): 4.6
• Sample 2: n=24, Mean(pg/ml): 2664, Standard deviation: 151.8, CV(%): 5.7
• Sample 3: n=24, Mean(pg/ml): 3724, Standard deviation: 242.1, CV(%): 6.5

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour HMOX1 Kit ELISA (ABIN2859305)

Huang, Zhu, Li, Sui, Min: "Effect of Blueberry Anthocyanins Malvidin and Glycosides on the Antioxidant Properties in Endothelial Cells." dans: Oxidative medicine and cellular longevity, Vol. 2016, pp. 1591803, (2017) (PubMed).

Détail du antigène

Antigène: HMOX1 (Heme Oxygenase (Decycling) 1 (HMOX1))

Autre désignation: HMOX1 (HMOX1 Produits)

Synonymes: HMOX1 Kit ELISA, ARABIDOPSIS THALIANA HEME OXYGENASE 1 Kit ELISA, ATHO1 Kit ELISA, F18A8.4 Kit ELISA, F18A8_4 Kit ELISA, GENOMES UNCOUPLED 2 Kit ELISA, GUN2 Kit ELISA, HEME OXYGENASE Kit ELISA, HEME OXYGENASE 1 Kit ELISA, HEME OXYGENASE 6 Kit ELISA, HO1 Kit ELISA, HY1 Kit ELISA, HY6 Kit ELISA, PLASTID HEME OXYGENASE Kit ELISA, REVERSAL OF THE DET PHENOTYPE 4 Kit ELISA, HO-1 Kit ELISA, wu:fc27c04 Kit ELISA, zgc:65984 Kit ELISA, D8Wsu38e Kit ELISA, Hemox Kit ELISA, Hmox Kit ELISA, Hsp32 Kit ELISA, HEOXG Kit ELISA, Heox Kit ELISA, Ho-1 Kit ELISA, Ho1 Kit ELISA, hsp32 Kit ELISA, MGC132176 Kit ELISA, Hmox1 Kit ELISA, HMOX1D Kit ELISA, HSP32 Kit ELISA, bK286B10 Kit ELISA, heme oxygenase 1 Kit ELISA, Plant heme oxygenase (decyclizing) family protein Kit ELISA, Heme oxygenase Kit ELISA, heme oxygenase Kit ELISA, heme oxygenase 1, chloroplastic Kit ELISA, heme oxygenase 1a Kit ELISA, heme oxygenase 1 L homeolog Kit ELISA, HMOX1 Kit ELISA, TED4 Kit ELISA, ho1 Kit ELISA, P9301_RS17555 Kit ELISA, A9601_RS17590 Kit ELISA, MAE_RS06565 Kit ELISA, HO1 Kit ELISA, hmox1a Kit ELISA, CPE0214 Kit ELISA, Cyan7425_2962 Kit ELISA, Hmox1 Kit ELISA, hmox1.L Kit ELISA, AM1_C0205 Kit ELISA, CC1G_11686 Kit ELISA, CpipJ_CPIJ007246 Kit ELISA

Sujet:

Protein Function: Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis.

Background: HMOX1 (heme oxygenase (decycling) 1) is a human gene that encodes for the enzyme heme oxygenase 1. It is localized to chromosome 22. Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family.

Synonyms: Heme oxygenase 1,HO-1,1.14.99.3,HMOX1,HO, HO1,

Full Gene Name: Heme oxygenase 1

Cellular Localisation: Microsome . Endoplasmic reticulum membrane, Peripheral membrane protein, Cytoplasmic side.

ID gène: 3162

UniProt: P09601

Pathways: Transition Metal Ion Homeostasis, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, SARS-CoV-2 Protein Interactome