Chemerin Kit ELISA

N° du produit ABIN2859335

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain Chemerin. Il a été cité dans 1 publication.

Aperçu rapide pour Chemerin Kit ELISA (ABIN2859335)

Antigène: Chemerin (RARRES2) (Retinoic Acid Receptor Responder (Tazarotene Induced) 2 (RARRES2))

Épitope: AA 17-163

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 78 pg/mL - 5000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Détail du produit Chemerin Kit ELISA

Seuil minimal de détection: 78 pg/mL

Fonction: Sandwich Human Chemerin/RARRES2 ELISA Kit to quantitate Human RARRES2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Analytical Method: Quantitative

Specificité: Expression system for standard: E.coli
Immunogen sequence: V17-S163

Capture antibody and Detection antibody: polyclonal antibody from goat, polyclonal antibody from goat

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 20 pg/mL

Ingrédients:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Précision du teste: Intra-Assay Precision (CV%): 4.7%, 6.4%, 7.6%
Inter-Assay Precision (CV%): 6%, 8.1%, 9.3%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Date de péremption: 12 months

Références pour Chemerin Kit ELISA (ABIN2859335)

Motawi, Mahdy, El-Sawalhi, Ali, El-Telbany: "Serum levels of chemerin, apelin, vaspin, and omentin-1 in obese type 2 diabetic Egyptian patients with coronary artery stenosis." dans: Canadian journal of physiology and pharmacology, Vol. 96, Issue 1, pp. 38-44, (2018) (PubMed).

Détail du antigène

Antigène: Chemerin (RARRES2) (Retinoic Acid Receptor Responder (Tazarotene Induced) 2 (RARRES2))

Autre désignation: RARRES2

Sujet:

Background: Chemerin, also known as RARRES2 or TIG2, is a protein that in humans is encoded by the RARRES2 gene. It is mapped to 7q36.1. Chemerin is a potent chemoattractant specific for antigen-presenting cells that requires proteolytic activation and acts as a ligand for the G protein-coupled receptor CMKLR1 (also known as ChemR23). It is a 14 kDa protein secreted in an inactive form as prochemerin and is activated through cleavage of the C-terminus by inflammatory and coagulation serine proteases. Chemerin was found to stimulate chemotaxis of dendritic cells and macrophages to the site of inflammation. What's more, the active protein has several roles, including that as an adipokine, and is truncated on both termini from the proprotein.

Gene Full Name: retinoic acid receptor responder 2

ID gène: 5919

UniProt: Q99969

Pathways: Brown Fat Cell Differentiation