Syndecan 1 Kit ELISA

N° du produit ABIN2859341

Détail du produit Syndecan 1 Kit ELISA

Antigène: Syndecan 1 (SDC1)

Épitope: AA 23-254

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156-10.000 pg/mL

Seuil minimal de détection: 156 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human SDC1/Syndecan-1

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: Q23-G254

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: Q23-G254

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Belongs to the syndecan proteoglycan family.

Plaque: Pre-coated

Protocole: human Syndecan-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Syndecan-1 has been precoated onto 96-well plates. Standards(NSO, Q23-G254) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Syndecan-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Syndecan-1 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Syndecan-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Syndecan-1 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(ng/ml): 1.23, Standard deviation: 0.048, CV(%): 3.9
• Sample 2: n=16, Mean(ng/ml): 3.25, Standard deviation: 0.15, CV(%): 4.6
• Sample 3: n=16, Mean(ng/ml): 5.87, Standard deviation: 0.299, CV(%): 5.1,
• Sample 1: n=24, Mean(ng/ml): 1.32, Standard deviation: 0.07, CV(%): 5.3
• Sample 2: n=24, Mean(ng/ml): 4.05, Standard deviation: 0.235, CV(%): 5.8
• Sample 3: n=24, Mean(ng/ml): 6.42, Standard deviation: 0.417, CV(%): 6.5

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: Syndecan 1 (SDC1)

Autre désignation: SDC1 (SDC1 Produits)

Synonymes: CG10497 Kit ELISA, CT27296 Kit ELISA, Dmel\\CG10497 Kit ELISA, Dsyn Kit ELISA, Syd Kit ELISA, Syn Kit ELISA, dSdc Kit ELISA, l(2)10608 Kit ELISA, sdc Kit ELISA, syd Kit ELISA, syn Kit ELISA, Syn1 Kit ELISA, cd138 Kit ELISA, synd1 Kit ELISA, xSyn1 Kit ELISA, syndecan1 Kit ELISA, Syndecan-1 Kit ELISA, XSyndecan-1 Kit ELISA, SDC1 Kit ELISA, CD138 Kit ELISA, SDC Kit ELISA, SYND1 Kit ELISA, syndecan Kit ELISA, AA408134 Kit ELISA, AA409076 Kit ELISA, Sstn Kit ELISA, Synd Kit ELISA, Synd1 Kit ELISA, syn-1 Kit ELISA, HSPG Kit ELISA, SYNDECA Kit ELISA, Syndecan Kit ELISA, Syndecan Kit ELISA, syndecan 1 Kit ELISA, Sdc Kit ELISA, sdc1 Kit ELISA, SDC1 Kit ELISA, Sdc1 Kit ELISA

Sujet:

Protein Function: Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix.

Background: Syndecan 1, also known as SYND1 or CD138, is a protein which in humans is encoded by the SDC1 gene. The protein encoded by this gene is a transmembrane(type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. This gene is mapped to 2p24.1. The syndecans mediate cell binding, cell signaling, cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan 1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. What’s more, Syndecan-1 is also a sponge for growth factors, with binding largely via heparan sulfate chains.

Synonyms: Syndecan-1,SYND1,CD138,SDC1,SDC,

Full Gene Name: Syndecan-1

Cellular Localisation: Membrane, Single-pass type I membrane protein. Secreted. Shedding of the ectodomain produces a soluble form.

ID gène: 6382

UniProt: P18827

Pathways: Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Lipid Metabolism